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comment by thundara
thundara  ·  2618 days ago  ·  link  ·    ·  parent  ·  post: Pubski: September 27, 2017

I made an embryoid body!

~2 months until I have the final cell type I'm looking at.





Cumol  ·  2617 days ago  ·  link  ·  

If you need any EBs blabla, let me know, we have loads of this stuff frozen :D

What do you want to diff. them into?

thundara  ·  2617 days ago  ·  link  ·  

Do they freeze alright? Their parent cells are so finicky that somehow I doubt mine would be happy with a drop of DMSO in solution.

And making microglia:

Cumol  ·  2617 days ago  ·  link  ·  

I just asked my colleague, they don't freeze the EBs but the next step in their protocol (neural crest like cells, the ones that surround the EBs after a few days in culture).