The bombing is the subject of a famous anti-war painting by Pablo Picasso. It was also depicted in a woodcut by the German artist Heinz Kiwitz, who was later killed fighting in the International Brigades. The bombing shocked and inspired many other artists, including a sculpture by René Iché, one of the first electroacoustic music pieces by Patrick Ascione, a musical composition by René-Louis Baron and poems by Paul Eluard (Victory of Guernica), and Uys Krige (Nag van die Fascistiese Bomwerpers) (English translation from the Afrikaans: Night of the Fascist Bombers). There is also a short film from 1950 by Alain Resnais entitled Guernica.

The painting is the background tapestry of the UN wall where world leaders walk into the security council.

On 5 February 2003 a large blue curtain was placed to cover this work at the UN, so that it would not be visible in the background when Colin Powell and John Negroponte gave press conferences at the United Nations. On the following day, it was claimed that the curtain was placed there at the request of television news crews, who had complained that the wild lines and screaming figures made for a bad backdrop, and that a horse's hindquarters appeared just above the faces of any speakers. Some diplomats, however, in talks with journalists claimed that the Bush Administration pressured UN officials to cover the tapestry, rather than have it in the background while Powell or other US diplomats argued for war on Iraq. In a critique of the covering, columnist Alejandro Escalona hypothesized that Guernica's "unappealing ménage of mutilated bodies and distorted faces proved to be too strong for articulating to the world why the US was going to war in Iraq", while referring to the work as "an inconvenient masterpiece."