Yeah, considering how ubiquitous antibody-based conclusions are in biology, I'm surprised there's not a more thorough creation and validation process. I straight up do not trust polyclonal antibodies (it's not a defined reagent!) and vendors have almost zero standards for showing specificity. I'm looking at ~20 targets to validate, and even if I could find antibodies for them all (many do not have mouse-specific versions), the process of formally checking antibody would be close to half a year of work. If I have my way though, "Mass Spec" will be Aim 1 of my thesis proposal, "Staining and Validation" will be Aim 2, "Drugging" will be Aim 3, and "Cell Culture Follow-up" will maybe be Aim 4. I'm hoping the results are exciting enough that doing the first two and doing them well will be sufficient for my (hypothetical) committee.Seems like biology 101, but the frequency with which it happens is astounding