This week I am basically a pipetting monkey. Preparing my samples for single cell sequencing. Probably the most important (and most expensive) experiment in my thesis. The results will basically decide my next two years and I hope that the results are good. I also attempted this with 70 cells a few months ago just to find out that its all junk (because of RNA degradation). I troubleshooted everything and optimized some things and until not it is looking fine. Lets hope I am not cross-contaminating my samples and I am not amplifying some bacteria... It has been the material for many dreams and nightmares for the past months and I guess it will not stop any time soon. Apart from that I am running, climbing and trying to enjoy the rest of the summer. Temperatures are already heading downwards and my head started to play Alex Clare and Imogen Heap every morning. Probably triggered by the way the air smells.